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rabbit anti ctb polyclonal antibody  (Aviva Systems)


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    Structured Review

    Aviva Systems rabbit anti ctb polyclonal antibody
    Rabbit Anti Ctb Polyclonal Antibody, supplied by Aviva Systems, used in various techniques. Bioz Stars score: 93/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+ctb+polyclonal+antibody/Anti-CD45%2FLCA+Rabbit+Polyclonal+Antibody+(OAAI00020)/us12059454-155-18-25
    Average 93 stars, based on 35 article reviews
    rabbit anti ctb polyclonal antibody - by Bioz Stars, 2026-10
    93/100 stars

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    Incubation:

    Article Title: Oral delivery of angiotensin converting enzyme 2 (ACE2) or angiotensin-(1-7)-bioencapsulated in plant cells attenuates pulmonary hypertensions, cardiac dysfunction and development of autoimmune and experimentally induced ocular disorders
    Article Snippet: After washing the plates with 1×PBST, homogenate plant protein was diluted to concentration of 0.1 μg/μL with the same plant extraction buffer and incubated in the GM1 coated plates overnight at 4° C., along with CTB (1 ng/Sigma), bovine serum albumin (1% w/v BSA) and untransformed wild type plant protein (0.1 μg/μL) as controls. .. The plates were blocked with PTM for one and half hr at 37° C. After discarding the PTM, rabbit anti-CTB polyclonal antibody (1:10,000 in PTM; GenWay) was incubated overnight at 4° C. Following washing three times with 1×PBST, goat anti-rabbit IgG-HRP secondary antibody (1:4,000 in PTM; Southern Biotechnology) was incubated for one and half hr at 37° C. The plates were washed with PBST thrice and with PBS once and 100 μL of tetramethyl benzidine (TMB) solution substrate (American Qualex Antibodies UCFL-05801) was added to the wells and incubated under dark for 5 min. .. The reaction was stopped by adding 50 μL of 2N H2SO4, and read the absorbance at 450 nm using plate reader (Bio-rad Model 680).

    Article Title: Oral delivery of angiotensin converting enzyme 2 (ACE2) or angiotensin-(1-7)-bioencapsulated in plant cells attenuates pulmonary hypertension, cardiac dysfunction and development of autoimmune and experimentally induced ocular disorders
    Article Snippet: .. Ninety-six-well plates were coated with serially diluted CTB standard (25-12.5-6.25-3.13-1.56-0.781-0.391-0.195 pg/μL; Sigma C9903) and CTB-ACE2 proteins (4,000-8,000-16,000) in bicarbonate buffer (15 mM Na2CO3, 35 mM NaHCO3, 3.08 mM NaN3, pH 9.6), then incubated overnight at 4° C. After washing the plates with 1× phosphate buffered saline (Fisher IC-N2810307) containing 0.05% (v/v) Tween 20 (1×PBST), the coated plates were blocked with 1×PBST containing 3% skim milk (PTM) for one and half hr at 37° C. and incubated with rabbit anti-CTB polyclonal antibody (1:10,000 in PTM; GenWay 18-511-245283) overnight at 4° C. The plates were followed by incubating with goat anti-rabbit IgG-HRP secondary antibody (1:4,000 in PTM; Southern Biotechnology 4030-05) for one and half hr at 37° C. after washing with 1×PBST thrice. .. The antibody-bound plates were washed with 1×PBST thrice and 1×PBS once before adding the 100 μL of tetramethyl benzidine (TMB) solution substrate (American Qualex Antibodies UCFL-05801).

    Article Title: Oral delivery of angiotensin converting enzyme 2 (ACE2) or angiotensin-(1-7)-bioencapsulated in plant cells attenuates pulmonary hypertensions, cardiac dysfunction and development of autoimmune and experimentally induced ocular disorders
    Article Snippet: .. Ninety-six-well plates were coated with serially diluted CTB standard (25-12.5-6.25-3.13-1.56-0.781-0.391-0.195 pg/μL; Sigma C9903) and CTB-ACE2 proteins (4,000-8,000-16,000) in bicarbonate buffer (15 mM Na2CO3, 35 mM NaHCO3, 3.08 mM NaN3, pH 9.6), then incubated overnight at 4° C. After washing the plates with 1× phosphate buffered saline (Fisher IC-N2810307) containing 0.05% (v/v) Tween 20 (1×PBST), the coated plates were blocked with 1×PBST containing 3% skim milk (PTM) for one and half hr at 37° C. and incubated with rabbit anti-CTB polyclonal antibody (1:10,000 in PTM; GenWay 18-511-245283) overnight at 4° C. The plates were followed by incubating with goat anti-rabbit IgG-HRP secondary antibody (1:4,000 in PTM; Southern Biotechnology 4030-05) for one and half hr at 37° C. after washing with 1×PBST thrice. .. The antibody-bound plates were washed with 1×PBST thrice and 1×PBS once before adding the 100 μL of tetramethyl benzidine (TMB) solution substrate (American Qualex Antibodies UCFL-05801).

    Article Title: Oral delivery of angiotensin converting enzyme 2 (ACE2) or angiotensin-(1-7)-bioencapsulated in plant cells attenuates pulmonary hypertension, cardiac dysfunction and development of autoimmune and experimentally induced ocular disorders
    Article Snippet: After washing the plates with 1X PBST, homogenate plant protein was diluted to concentration of 0.1 μg/μL with the same plant extraction buffer and incubated in the GM1 coated plates overnight at 4° C., along with CTB (1 ng/μL, Sigma), bovine serum albumin (1% w/v BSA) and untransformed wild type plant protein (0.1 μg/μL) as controls. .. The plates were blocked with PTM for one and half hr at 37° C. After discarding the PTM, rabbit anti-CTB polyclonal antibody (1:10,000 in PTM; GenWay) was incubated overnight at 4° C. Following washing three times with 1X PBST, goat anti-rabbit IgG-HRP secondary antibody (1:4,000 in PTM; Southern Biotechnology) was incubated for one and half hr at 37° C. The plates were washed with PBST thrice and with PBS once and 100 μL of tetramethyl benzidine (TMB) solution substrate (American Qualex Antibodies UCFL-05801) was added to the wells and incubated under dark for 5 min. .. The reaction was stopped by adding 50 μL of 2N H2SO4, and read the absorbance at 450 nm using plate reader (Bio-rad Model 680).

    Saline:

    Article Title: Oral delivery of angiotensin converting enzyme 2 (ACE2) or angiotensin-(1-7)-bioencapsulated in plant cells attenuates pulmonary hypertension, cardiac dysfunction and development of autoimmune and experimentally induced ocular disorders
    Article Snippet: .. Ninety-six-well plates were coated with serially diluted CTB standard (25-12.5-6.25-3.13-1.56-0.781-0.391-0.195 pg/μL; Sigma C9903) and CTB-ACE2 proteins (4,000-8,000-16,000) in bicarbonate buffer (15 mM Na2CO3, 35 mM NaHCO3, 3.08 mM NaN3, pH 9.6), then incubated overnight at 4° C. After washing the plates with 1× phosphate buffered saline (Fisher IC-N2810307) containing 0.05% (v/v) Tween 20 (1×PBST), the coated plates were blocked with 1×PBST containing 3% skim milk (PTM) for one and half hr at 37° C. and incubated with rabbit anti-CTB polyclonal antibody (1:10,000 in PTM; GenWay 18-511-245283) overnight at 4° C. The plates were followed by incubating with goat anti-rabbit IgG-HRP secondary antibody (1:4,000 in PTM; Southern Biotechnology 4030-05) for one and half hr at 37° C. after washing with 1×PBST thrice. .. The antibody-bound plates were washed with 1×PBST thrice and 1×PBS once before adding the 100 μL of tetramethyl benzidine (TMB) solution substrate (American Qualex Antibodies UCFL-05801).

    Article Title: Oral delivery of angiotensin converting enzyme 2 (ACE2) or angiotensin-(1-7)-bioencapsulated in plant cells attenuates pulmonary hypertensions, cardiac dysfunction and development of autoimmune and experimentally induced ocular disorders
    Article Snippet: .. Ninety-six-well plates were coated with serially diluted CTB standard (25-12.5-6.25-3.13-1.56-0.781-0.391-0.195 pg/μL; Sigma C9903) and CTB-ACE2 proteins (4,000-8,000-16,000) in bicarbonate buffer (15 mM Na2CO3, 35 mM NaHCO3, 3.08 mM NaN3, pH 9.6), then incubated overnight at 4° C. After washing the plates with 1× phosphate buffered saline (Fisher IC-N2810307) containing 0.05% (v/v) Tween 20 (1×PBST), the coated plates were blocked with 1×PBST containing 3% skim milk (PTM) for one and half hr at 37° C. and incubated with rabbit anti-CTB polyclonal antibody (1:10,000 in PTM; GenWay 18-511-245283) overnight at 4° C. The plates were followed by incubating with goat anti-rabbit IgG-HRP secondary antibody (1:4,000 in PTM; Southern Biotechnology 4030-05) for one and half hr at 37° C. after washing with 1×PBST thrice. .. The antibody-bound plates were washed with 1×PBST thrice and 1×PBS once before adding the 100 μL of tetramethyl benzidine (TMB) solution substrate (American Qualex Antibodies UCFL-05801).



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